fracture association results were also obtained for the 82 most significant SNPs from 54,244 individuals of European ancestry from 7 GWAS (in-silico genotyping) that had not been included in the stage 1 analyses (Supplementary Tables 19A, 19B and 19C). Subjects from 34 studies of the GENOMOS consortium with BMD and/or fracture information were studied for replication (Supplementary Tables 3A, 3B and 3C). De-novo replication genotyping was done in the UK (Kbiosciences), Iceland (deCODE Genetics), Australia (University of Queensland Diamantina Institute) and the USA (WHI GeCHIP) using KASPar, Centaurus, OpenArray and iSelect assays respectively (Supplementary Note). Minimum genotyping quality control criteria were defined as: Sample call rate > 80%, SNP call rate > 90%, HWE P > 1×10−4, MAF > 1%.
