Given the importance of location for neuronal function, we wanted to assign a spatial distribution to each cell type. The Allen Mouse Brain Atlas provides systematic high-quality information about gene expression based on ISH. The data are available both as images and in the form of three-dimensional volumetric maps. We computed the spatial extent of each cell type by correlating volumetric and RNA-seq gene expression using only cell-type-specific genes as determined by a significant enrichment score (STAR Methods). The resulting data were visualized as three-dimensional density maps, expected to peak in regions where each cell type was abundant.
