We assessed cytokine and chemokine profiles released in the supernatant of pMGL cultures. Unstimulated pMGLs secreted detectable levels of various cytokines and chemokines, including IL-8, CXCL1 or CCL2 (Fig. 4a, b). Furthermore, the addition of IFN-γ and endotoxin (LPS) triggered the release of chemokine and cytokines above baseline (Fig. 4a–d). In particular, CXCL10, MIP1a, IL-6 and TNFα were increased more than 2-fold within the dynamic range of this assay. We verified that this effect was detectable at the transcriptional level, with an increase in mRNA of IL-6 and TNFα (Fig. 4c, d).
