Considered together, these data confirm a key role for DPY30 during germ layer specification while suggesting that the requirement for DPY30 expression could vary during the differentiation and maturation of specific lineages (Fig. 6E). Overall, these experiments illustrate how the optimized inducible knockdown platform can be easily applied to acquire novel information about developmental mechanisms by performing functional studies at different steps of hPSC differentiation into multiple cell types.
