KIAA1212 also directly bind to DISC1 (Figure 2D). We further examined the affinity between the CT2 domain of KIAA1212 and DISC1 in vitro (Figure 2E). Dose-response analysis estimated a dissociation constant (Kd) at 1.0 μM, which is very similar to the reported Kd between KIAA1212 and actin (Enomoto et al., 2005). It is possible that the affinity between the full length KIAA1212 protein and DISC1 is much higher, given that multiple domains of KIAA1212 showed direct association with DISC1.
