Immunohistochemistry showed staining for ZNF804A in human cerebral cortex sections (Figure 4B-F), especially in lamina III and concentrated over pyramidal neurons. Immunoreactivity was strong in the cytoplasm and proximal dendrites, often with a prominent granular appearance (Figure 4D-F). Some staining of nuclei was also observed. Other cell populations did not show consistent immunolabeling. Findings were similar in all cortical regions examined and were comparable between free-floating and slide-mounted sections. The pyramidal cell immunoreactivity was abolished by preincubation with the blocking peptide (eFigure 5 in the Supplement).
