Hippocampal slices were prepared as described above. Following a baseline recording, slices were either given control stimulation (3 pulses/min) or TBS. All the slices were collected 7 minutes after stimulation and placed into cold 4% paraformaldehyde. As previously described53, hippocampal slices were subsection of a freezing microtome and slide mounted. Immunocytochemical labeling was done by washing slices with 0.1M phosphate buffer, then placed in a cocktail of primary antibodies pCofilin (ABCam, AB12866; 1:250) and PSD95 (ThermoScientific, MA1-045, 1:800) in diluent which included 0.1M PB, 0.3% Triton, and 1.8% bovine serum albumin. Sections were exposed to primary antibody for two nights at 4°C. Tissue was then rinsed 3 times with 0.1M PB and incubated in secondary antibodies for donkey anti-mouse Alexafluor 488 (Life Technologies; A-21202) and donkey anti-rabbit Alexafluor 594 (Life Technologies; A-2107).
