As the mice treated with D-Lys3-GHRP-6 clearly showed a reduced preference for alcohol when compared to the saline-treated animals, we next tested the effect of this drug in a single-bottle DID procedure known to produce substantial blood ethanol concentrations and ethanol-induced c-Fos expression. Mice were injected with saline for three days as before but allowed to drink only 20 % ethanol for 2 h (having access to water throughout the rest of the day) and then on day 7, either saline or D-Lys3-GHRP-6 was injected and the mice allowed to drink 20 % ethanol for 4 h. As shown in Fig. 2A, ethanol intakes were high during the 2 h access period: on day 4, the mice ingested 2.81 ± 0.20 g/kg, on day 5, 2.45 ± 0.21 g/kg and on day 6, 2.63 ± 0.16 g/kg. Mice injected with D-Lys3-GHRP-6 showed a significantly reduced intake of alcohol (2.49 ± 0.32 g/kg) when compared to the saline-injected mice (4.99 ± 0.38) [F(1,27) = 24.87, p < 0.0001] during the 4 h access period. The resulting blood ethanol concentrations after D-Lys3-GHRP-6
