Chunk #19 — Results and Discussion — Gene expression differences between isolation methods are associated with cellular composition and B-cell manipulation
Because PLS-DA analysis only gives an overview of variation for probes across all methods, we refined our GO analysis by making pair-wise comparisons of closely related isolation methods focusing on i) uniquely detected probes and ii) overlapping detected probes that were significantly differentially expressed between two methods (Figure 5). In this analysis, we ranked the probes on significance and then selected 5% of the most significant probes that showed an at least three-fold change.
