Chunk #20 — Results and Discussion — Gene expression differences between isolation methods are associated with cellular composition and B-cell manipulation
In the PAX-PBMC comparison, 456 probes were detected in PAX but not in the PBMCs and 3,507 probes vice versa. For the uniquely detected probes in the PBMCs and PAX, none of the GO terms was significantly enriched after FDR correction. We found 374 (4.5%) probes differentially expressed between PAX and the PBMCs (Table 3). These probes showed an enrichment of "gas transport" (GO:0015669) containing genes CA2, HBD and HBQ1 in PAX (Table 4). The GO term "Macromolecule biosynthetic process" (GO:0009059) was most significantly enriched in the PBMCs containing 33 genes (including GYPC, RPL26L1, EEF1B2, RPS27A, MTIF2) encoding proteins such as ribosomal proteins, translation initiation and elongation factors.
