The gene-based tests of associations were run on 24 576 genes/genetic regions (see ‘Materials and Methods' section for details). The Manhattan and QQ plot for this test are shown in Figures 1a and b. Results for the top 100 genes can be found in Supplementary Table 5. As shown in Table 3, four genes and one intergenic noncoding RNA region were significantly (false discovery rate-corrected P<0.05) associated with lifetime cannabis use: (i) neural cell adhesion molecule 1 (NCAM1, on 11q23); (ii) cell adhesion molecule 2 (CADM2, on 3p12); (iii) short coiled-coil protein (SCOC) and (iv) SCOC antisense RNA1 (SCOC-AS1, both located on 4q31); and (v) potassium channel, subfamily T, member 2 (KCNT2, on 1q31). Regional plots44 of these top genes are located in Supplementary Figure 6.
