Effects of HFD on the levels of OPN, F4/80, TNFα, and MCP-1(A) Whole liver lysates were used to evaluate the levels of OPN (upper panel), cleaved OPN (middle panel), or the loading control p38 (lower panel) by immunoblot analyses. (B–E) F4/80 positive cells were also determined in all four groups using immunohistochemistry. Arrows indicate F4/80 positive cells. (F) The number of F4/80 positive cells was evaluated in at least five high power fields (HPF) (inset in D). Intrahepatic levels of TNFα (G) and MCP-1 (H) determined by ELISA are shown. *Significantly different from all other groups for Figures (F,H). #Significantly different from corresponding null group for Figure (H).
