Functional clustering using the Ingenuity Pathway Analysis (IPA) software was performed to determine common processes (within the subset of neurogenesis) affected by moderate PAE. All genes altered by PAE for each set of culture conditions were analyzed together. Tables 1 and 2 show results from the analysis for the proliferating and differentiated culture conditions, respectively, indicating the most significant gene ontology (GO) categories and functional annotations within those categories altered by PAE. Gene clusters derived from genes altered by PAE indicate that alcohol influences nervous system development and function, cell death and survival, cellular movement, and embryonic development, cellular function and maintenance, and behavior. The functional annotations within the GO categories for genes altered under proliferating conditions are particularly interesting: they include the quantity and migration of neuroglia and neurons, neuritogenesis and outgrowth of neurites, long-term potentiation, neuronal apoptosis, behavior, blood vessel development, proliferation, and organization of the cytoskeleton, all with p <.001 as seen in Table 1. These categories mostly include Adora2a and Vegfa. Functional annotations for genes altered under differentiated conditions include survival of neurons; differentiation of NPCs,
