Mixed-effects models examining RT-qPCR GABRA2 RNA levels revealed a significant effect of GABRA2 rs279858 genotype (F=3.7, df=2,15.8, p=0.046). Neural cell cultures derived from rs279858*C-allele donor subjects had lower levels of GABRA2 RNA than T-allele homozygotes, Figure 1A (T/T vs. C/T F=4.9, df=1,20, p=0.039, T/T vs. C/C F=6.1, df=1,12.1, p=0.03, C/C vs. C/T F=0.10, df=1,10.8, p=0.76). In contrast, there was no significant effect of rs279858 genotype on RNA expression of RBFOX3 (Figure 1B, F=0.562, df=2,19.3, p=0.58), which encodes the neuron-specific transcription factor NeuN, suggesting that the between-sample differences in GABRA2 expression were not due to variability in the capacity of iPSC lines to differentiate into neurons.
