The effects of different alleles of rs1229982 and rs1159918, along with the other natural variations, were tested by transient transfection assays. The region was amplified from DNA of different individuals to generate the natural haplotypes, and cloned into the pXP2 reporter plasmid. Five different haplotypes (Table 2) were obtained; all five had the same allele for SNPs rs28913901, rs28913903, rs28913904, rs3076071 and rs28913905 (which have low minor allele frequencies); they differed in the remaining seven positions (Figure 1).
