Chunk #13 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.1. Seeding NPCs for differentiation — Day-3: Preparing coated plates (or coverslips)

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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For multiple electrode array (MEA) assays, plates were first coated with poly-L-ornithine (Sigma #P4957, 10 μg/ml in dH2O) overnight at 37 °C. Following three washes with dH2O, Laminin (ThermoFisher Scientific, #23017-015, 1 μg/ml in 1 × PBS) was added again overnight at 37 °C. For confocal microscopy of mNgn2-induced neurons, sterile pre-etched coverslips (Fisher Scientific, #12-545-80) were coated the same way but with 50 μg/ml poly-L-ornithine.