Chunk #14 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.1. Seeding NPCs for differentiation — Day-2: Seeding NPCs

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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We recommend that 1 μg/ml Laminin be included in both the NPC medium and neuron medium (Table 1) to promote neurite growth and cell attachment during neuronal induction [34].