Chunk #15 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.1. Seeding NPCs for differentiation — Day-2: Seeding NPCs

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Prior to beginning, NPC medium, DMEM and Accutase were pre-warmed. The coating mixture was aspirated and 230 μl of NPC medium was aliquoted into each well of the 24-well plate well.