was used for data acquisition and analysis. GIRK currents were confirmed by inhibition with Ba2+ (1mM), a selective inhibitor of inward rectifiers. For the GTPγS experiment, GTPγS (0.1 µM) was added to the internal solution in place of Na3GTP. For recording spontaneous IPSCs, the internal solution contained (in mM): 130 CsCl, 4 NaCl, 2 MgCl2, 1.1 EGTA, 5 HEPES, 2 Na2ATP, 5 sodium creatine-phosphate, 0.6 Na3GTP. Currents were filtered at 2kHz and digitized at 10kHz. Following whole-cell access, cells were held at −60mV and spontaneous activity recorded for 5 minutes per cell. At the end of recording for at least one cell per genotype, 50µM bicuculline was added to ensure GABAAR specificity.
