The following primary antibodies were used for immunohistochemistry (IHC): Rabbit monoclonal anti-Ki-67 (1:1000; LabVision), mouse anti-BrdU (1:100; Becton Dickinson), goat polyclonal anti-DCX (1:700; Santa Cruz Biotechnology), and rabbit polyclonal anti-activated caspase-3 (AC-3; 1:500; Cell Signaling Technology). The left and right hemisphere of every ninth section through the mPFC and hippocampus were slide-mounted and dried overnight prior to IHC. Slides were coded prior to IHC, and the code was not broken until after analysis was complete. All incubations were performed at room temperature unless otherwise indicated. Slide-mounted sections were subjected to pretreatment steps as described previously (Mandyam et al., 2004). Slides were incubated with 0.3% H2O2 for 30 min to remove any endogenous peroxidase activity. Nonspecific binding was blocked with 5% serum and 0.5% Tween-20 in 0.1M PBS for 60 min and incubated with the primary antibody (in 5% serum and 0.5% Tween-20) for 18-20 h. After washing with 0.1M PBS, the sections were exposed to biotinylated secondary IgG for 1 h (1:200; Vector Laboratories). After secondary antibody incubation, slides were incubated in ABC for 1 h (Vector Laboratories), and staining
