Together, the above data indicate that reduced BAF53b function interferes significantly with the functional and structural foundations of long-term memory, i.e., memory consolidation. Therefore we set out to identify which genes BAF53b regulates during memory consolidation. We performed an RNA Sequencing experiment (Fig. 7) from dorsal hippocampal tissue from four groups of animals: Baf53b+/− homecage (Baf53b+/− HC); Baf53b+/− behavior (Baf53b+/− Beh); wildtype homecage (WT HC); and wildtype behavior (WT Beh). All Baf53b+/− and wildtype animals were handled and habituated as described for OLM (Fig. 2A, supplemental Fig S2). On the training day, half the animals from each genotype were sacrificed 30min after OLM training (Beh) or directly from their home cage without training (HC). We have previously observed significant gene expression changes in the dorsal hippocampus 30 min following OLM training28. Mean PHRED quality scores indicate high quality sequencing data for each replicate (Supplemental FigS6A). After mapping and considering the haploid genome29,30, RNA Sequencing successfully covered transcriptome 298.50 times (Supplemental Fig S6B) and significant differences in expression profiles were examined between all pairs of samples for p<0.0531-33.
