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Chunk #142 — STAR★Methods — Quantification and Statistical Analysis — Marker gene set discovery

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Molecular Architecture of the Mouse Nervous System.
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We generated gene sets in this manner for all clusters, with up to six genes per cluster. We also calculated the cumulative selectivity, specificity (difference between the posterior probability for the best cluster and that of the second-best cluster), and robustness (the posterior probability that all genes would be detected in the cluster, based on trinarization scores). We reported these statistics cumulatively for n = 1, 2, 3, 4, 5 and 6 genes. Generally, robustness drops as more genes are added, while selectivity increases. Specificity tends to increase as the gene set becomes more selective, but then decrease as it becomes less robust.