Figure 1 shows that gene expression profiles of hiPSC-derived neural cells most closely recapitulate those of the fetal forebrain. We compared hiPSC forebrain NPC and 6-week-old neuron microarray gene expression profiles to the Allen BrainSpan: Atlas of the Developing Human Brain (http://www.brainspan.org) using Spearman Rank Correlation analysis, and found that control and SZ hiPSC forebrain NPCs and 6-week-old neurons shared the most similarity with first trimester fetal brain tissue (Figure 1a and Supplementary Figure 1). Heatmaps generated through a Wilcoxon's rank-sum test revealed that the earliest time points of brain tissue (8–16 weeks post conception for hiPSC NPCs, 8–24 weeks post conception for hiPSC 6-week-old neurons) were consistently enriched with high significance, particularly in the cortical and subcortical forebrains and the ganglionic eminence. Control and SZ hiPSC NPCs and 6-week-old neurons shared highly similar spatial/temporal profiles relative to the Allen BrainSpan Atlas (Figure 1a); the correlation between control and SZ spatio-temporal similarity relative to the Allen Brain Atlas (−log10 p rank-sum test) for the NPCs was 0.9993 (P<1.4e–48), whereas for the 6-week-old neurons it was 0.9670 (P<9.1e–19). Each patient and
