Progeny carrying the Pparαflox/flox alleles (figure 1A), referred to as floxed, were backcrossed in the C57Bl/6J background, and then crossed with albumin-Cre mice in the same genetic background, generating a hepatocyte-specific PPARα knockout (Pparαflox/floxalbumin-Cre+/−) referred to as Pparαhep−/− (figure 1B). PPARα mRNA was not detected in livers from Pparαhep−/− mice when compared with floxed and C57Bl6/J mice (figure 1C), suggesting that most hepatic PPARα expression is from hepatocytes. PPARα absence in hepatocytes did not alter mRNA expression of other PPAR isotypes (figure 1C).
