Brain gene expression data are available for mice selectively bred for ethanol preference from F2 intercross, HS4, HS/NPT and HS/Ibg founders and for inbred laboratory strains and RI strains that differ in ethanol preference (or consumption) (Bubier et al. 2014; Hoffman et al. 2014; Metten et al. 2014; Mulligan et al. 2006; Williams & Mulligan 2012). However, as sequence data became available from multiple inbred mouse strains (Keane et al. 2011; Roberts et al. 2007), it has become clear that the preference/consumption studies have engaged only a fraction of the genetic diversity available in M. musculus. By increasing the genetic diversity, one has the potential to detect new pathways regulating ethanol preference and consequently to detect new targets for manipulation. With this perspective in mind, the current study was undertaken using HS-CC animals as the founders for short-term selective breeding. Three generations of breeding were sufficient for significant segregation of the high and low preference lines; preference in the high line was approximately 10 times greater than in the low line. Genotyping the lines not only confirmed the segregation of
