The DW and splicing network data were integrated to focus on those nodes that are hubs in only one of the selected lines and show a significant change in affected edges. Thirty-four genes met these criteria (Table S9). When compared with all DW genes, there are no significant functional, molecular or component GO annotation enrichments for this group of genes, nor was there a significant enrichment in one or more of the cosplicing modules. The number of significantly changing hubs in the high and low lines was equal. Several of the hub nodes have been directly associated with alcohol-related phenotypes: Tnik, Pde10a, Ndrg1, Sos1, Tmem208, Nploc4, Slc6a15 and Pcsk1 (Bell et al. 2009; Costin et al. 2013; Logrip & Zorrilla 2014; Parsons et al. 2012; Rodd et al. 2009). An additional 18 of the splicing hubs are associated with mouse preference QTL intervals (GeneWeaver.org) (Table S9).
