We generated a doxycycline-inducible, human codon optimized Cas9 (hCas9) construct contained within a piggyBac transposon7. The piggyBac transposon construct was stably introduced into the male PGP1-iPS cell line by co-transfecting it with a plasmid encoding the piggyBac transposase (Fig. 1b). The resulting PGP1-hCas9-PB stable cell line showed greater than 1000-fold induction of Cas9 by doxycycline (Dox) treatment (Fig. 1c).
