We reasoned that Cas9 genome editing efficiency could be enhanced by generating a stable cell line that harbors an inducible Cas9 transgene encapsulated on a piggyBac transposon (Fig. 1a). Genome editing is performed by Cas9 induction accompanied by transfection of gRNA and homology-directed repair DNA donor template. The Cas9 transgene can be removed by transient transfection with piggyBac transposase.
