Expression of mini-w has a negligible impact on ethanol sedation sensitivity and internal ethanol concentrations in ethanol sedation assaysA. Compiled ST50 values from ethanol sedation assays with vapor from 50% ethanol did not correlate with w+ rank in TGFβR (blue circles), Akap200 (green triangles) and Gal4 (red squares) strains (Pearson r=−0.1754, p=0.4125, n.s.). ST50 values are represented as fold of w1118 controls. B. Knockdown of mini-w in the nervous system and initial sensitivity to ethanol. Expression of w RNAi transgenes (v30033 and v30034) was driven in the nervous system by elav-Gal4. Genotype had a significant overall effect on ST50 values from ethanol sedation assays with vapor from 50% ethanol (one-way ANOVA, p=0.0008, n=8–16 per genotype). ST50 values in w1118, elav-Ga4/+, v30033/+ and v30034/+ genotypes were not statistically different (Bonferroni’s, n.s.). ST50 values in elav-Gal4;v30033 and elav-Gal4/v30034 knockdown animals were greater than in elav-Ga4/+ (Bonferroni’s, *p<0.05), but were not significantly different from v30033/+ or v30034/+ controls (Bonferroni’s, n.s.). C. Internal ethanol concentrations in nervous system mini-w knockdown flies after 30 minutes of exposure to vapor from 50% ethanol in ethanol sedation assays. Genotype had a significant overall effect on internal ethanol (one-way ANOVA; p=0.0388; n=4), but no differences between relevant genotype pairs were found (Bonferroni’s, n.s.).
