Nervous system knockdown of mini-w in flies with altered sensitivity to ethanolExpression of v30034 along with either Cnx14D RNAi v5597 (A) or ph-p RNAi v50024 (B) RNAi was driven in the nervous system by elav-Gal4. All flies tested were females. A. Knockdown of mini-w in the nervous system of in flies with decreased sedation in response to vapor from 50% ethanol. There was a significant overall effect of genotype on ST50s (one-way ANOVA, p<0.0001, n=8). ST50 values were not significantly different in w1118, v5597/+, elav-Gal4/+ or elav-Gal4,v30034/+ flies (Bonferroni’s, n.s.). elav-Gal4/v5597 and elav-Gal4,v30034/v5597 exhibited significantly higher ST50 values compared to relevant controls (*Bonferroni’s, p<0.05 compared to v5597/+ and elav-Gal4/+; **Bonferroni’s, p<0.05 compared to v5597/+ and elav-Gal4,v30034). elav-Gal4/v5597 and elav-Gal4,v30034/v5597 were not statistically distinguishable (Bonferroni’s, n.s.). B. Knockdown of mini-w in the nervous system in flies with increased sensitivity to sedation from vapor from 50% ethanol. Overall, genotype had a significant effect on ST50s (one-way ANOVA, p<0.0001, n=8). ST50 values were indistinguishable in w1118, elav-Gal4/+ and elav-Gal4,v30034/+, whereas the ph- v50024/+ control was significantly different from w1118 (#Bonferroni’s, p<0.05). elav-Gal4;v50024 and elav-Gal4/v30034;v50024 were not different from each other, but they were significantly different from their relevant controls (*Bonferroni’s, p<0.05 compared to elav-Gal4/+ and v50024/+; **Bonferroni’s, p<0.05 compared to elav-Gal4/+ and elav-Gal4,v30034).
