Chunk #11 — METHODS — BRET

Source: TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis.
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BRET assays were performed and analyzed as described by previously30. GFP2 and RLuc fusion protein constructs for BRET studies were generated by cloning TNFR1 sequences into pGFP2-N3 and pRLuc-N3 vectors (BioSignal Packard).