1H-MRS data was acquired by single voxel spectroscopy (SVS) using a point resolved spin echo (PRESS) sequence 128 averages; TR, 3000 ms; TE, 30 ms; voxel size was 15 mm × 15 mm × 15 mm. The region of interest was placed in the left DLPFC by the neuroradiologist (Figure 2). During the second spectroscopy, voxel localization parameters were copied and adjusted to the position of patient. Automated procedures were used to optimize radiofrequency pulse power, field homogeneity, and water suppression, as well as to convert the lines into a Gaussian shape. Spectroscopy data was processed by means of Avanto Syngo MR Software (Siemens AG, Munich, Germany), Level B15. The processing included: k-space Fourier transformation and a spatial 50 Hz Hanning filter; subtraction of the residual water signal; time domain 1 Hz exponential apodization; zero filling to 2048 points; Fourier transformation of the time domain signals; frequency shift correction, phase correction and baseline correction. The fitting error was automatically computed as a deviation between theoretical and measured spectrum determined using the last squares method. Values less than 0.5 were considered
