TD31 (Table S4), and their eigengenes were consistently upregulated across time points, in keeping with their developmental annotation. The brown and tan modules (annotated by “synaptic transmission” and “gated channel activity” GO terms, respectively), however, were enriched in upregulated genes only at TD31 (Table S4), and consistently, their eigengenes were upregulated more at TD31 than at TD11, in line with their synaptic functional annotation (Figure 2A). Hierarchical clustering of module eigengenes also showed a tighter correlation among the green and yellow, the blue and magenta, and the brown and tan modules (Figure 2B), suggesting similar functions and/or tighter regulatory interactions. In sum, probands were characterized on the transcriptome level by a downregulation of non-neuronal and a corresponding upregulation of neuronal transcript modules.
