We used the Illumina NOS and NOE datasets to analyze the effect of alcohol on the expression of hippocampal Cd14 gene in BXD mice using a two-way ANOVA with treatment and strain as the between subjects factors. The average of Cd14 expression across BXD strains in the Illumina NOS group was 7.378, while the average for the Illumina NOE group was 7.318, indicating a decrease in expression after ethanol treatment. Statistical analysis also showed a significant effect of treatment and strain on Cd14 transcript abundance (Table 3).