mAChRs are also present in the striatum (Hersch et al., 1994) and the mAChR agonist oxotremorine decreases DA release evoked by single pulse electrical stimulation, but increases release evoked by high frequencies (Threlfell et al., 2010). Furthermore, genetic deletion of the muscarinic M4 subunit prevents this modulation of DA release in NAc (Threlfell et al., 2010). However, in contrast to nAChRs, there is no evidence of M4 expression on DA terminals, suggesting that mAChR activation preferentially inhibits ACh release in the NAc (Threlfell et al., 2010; Threlfell and Cragg, 2011).
