Metten et al. (1998b) used short-term selective breeding (STSB) from a B6×D2 F2 intercross to produce what they termed short-term drinking response high and low lines and high/low acute withdrawal lines; the lines showed the expected reciprocal response for acute withdrawal and consumption. The current study used a similar experimental design except that HS mice were used as the founding population. The HS population was formed by crossing the B6, D2, BALB/cJ (C), and LP/J (LP) inbred strains. This HS population (hereafter termed HS4) has approximately twice the genetic diversity of a B6×D2 F2 intercross and more than three times the genetic diversity of the standard BXD RI panel (see Roberts et al. 2007). The C strain is intermediate between the B6 and D2 strains in terms of ethanol consumption and acute withdrawal (Metten and Crabbe 1994; Fehr et al. 2002). The LP is similar to the D2 strain in terms of ethanol consumption (low; Yoneyama et al. 2008); to our knowledge, there are no reports describing ethanol withdrawal in the LP strain. However, acute ethanol withdrawal in the closely
