We generated trans-heterozygous flies that express the nuclear-tagged GFP “INTACT” transgene (5xUAS-unc84-GFP) (Henry et al. 2012) in all MB neurons (MB010B-Gal4). Expression of INTACT was MB-specific (Supplemental Material, Figure S1, A and B) and did not disrupt odor-cue-induced ethanol memory (Figure S1C). MB-INTACT flies were subjected to one of four treatment paradigms—air, EtOH, odors, or trained—and flash frozen the following day (Figure 1A). MB nuclei were isolated and RNA-sequenced to a depth of ∼30 million reads per sample (Figure S2). Nearly all libraries showed >80% uniquely mapped reads and ∼15 million fragments per kilobase of transcript per million mapped reads (FPKM) per sample.
