RNA was isolated and converted to cDNA. Diluted cDNA (20×) was used to assess the expression of Fkbp5 in Amy, Acb, mPFC VTA, dStr, and CCx using CFX96 Touch Real-Time PCR Detection System real-time PCR (BioRad, USA) (Table S1). Relative gene transcripts levels in all animals of each experimental group were determined using the ∆CT method (Biorad real time PCR application guide) (Table S2; Fig. S2 and S3).
