The repeated binge paradigm had its major impact on the DRN. The CeA and Acb-sh of these same animals showed many fewer genes with altered expression, 309 in the central amygdala and 154 in the Acb-sh (McBride et al., 2014). Sixteen of the 22 altered pathways in the Acb-sh and 3 of the 4 in CeA were also altered in the DRN (noted in Table 4 and Supplementary Table 2). The large difference in numbers of altered genes might be in part attributable to the differences in the cell populations of these 3 regions; the DRN is more focused on the serotonin system, with nearly 50% of the neurons serotonergic (Waselus et al., 2011). More heterogeneity in the other regions could dilute the changes and make them more difficult to identify. Also, differences in the postnatal development of the DRN compared to the other regions may make it more vulnerable to ethanol. Another potential contributor to the difference among the regions is the different technology used to analyze those regions; the RNA sequencing used here may be more sensitive to
