Chunk #13 — Materials and Methods — Quantitative Reverse Transcription PCR (qRT-PCR) Analysis

Source

Genome-Wide DNA Methylation Profiling Reveals Epigenetic Changes in the Rat Nucleus Accumbens Associated With Cross-Generational Effects of Adolescent THC Exposure.

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mRNA levels for 13 genes were measured in the NAc of a separate cohort of THC and VEH animals (n=8 males and 8 females/group). The data were analyzed using the ΔΔCT method (Livak and Schmittgen, 2001), and statistical significance was assessed using the Welch two-sample t-test. More details are described in the Supplementary Information.