Chunk #14 — Results — Differentially Methylated Regions in the NAc Are Associated With Cross-Generational Effects of Adolescent THC Exposure in Adult F1 Offspring
To characterize cross-generational epigenetic signatures associated with adolescent Cannabis use, we compared genome-wide DNA methylation levels in the NAc of two groups of unrelated F1 Long-Evans rats born with F0 individuals that had either been exposed to THC or VEH during adolescence. From each ERRBS library (n=32), we generated a mean of 196 062 679 reads (50 bp single-end). An average of 93% of bases per library had quality (Q) scores >30, and >2.2 million CpGs were interrogated per individual genome (mean=3 454 502). Raw methylation files have been deposited in GEO (GSE69984). On the basis of PCA using autosomal CpG methylation profiles, two individuals were identified as outliers and were excluded from subsequent analyses (Supplementary Figure S3).
