Chunk #15 — Results — Differentially Methylated Regions in the NAc Are Associated With Cross-Generational Effects of Adolescent THC Exposure in Adult F1 Offspring
We first applied two important filtering criteria in an attempt to increase the reliability of data being analyzed at a given CpG and ultimately limit the occurrence of false-positives. First, we required high sequencing read depth per CpG per sample, filtering out loci with lower than 30-fold coverage, as the accuracy of methylation estimates at sites with lower read coverage are compromized by random sampling variation. For example, at 30-fold coverage, a single read at a given CpG accounts for 3% of the methylation estimate, in contrast to a site with 10-fold coverage at which a single read accounts for 10% of the estimate. In our data set, 758 204 autosomal CpGs met this requirement across all individuals, with an average read depth per CpG of 224 reads (min=49.5; max=585.7).
