Linear mixed regression analyses were performed to investigate the relationship between age, gender, cause of death, region, PMI, pH, RIN and present call (%P). Statistical analyses were conducted using Partek® Genomics Suite™ version 6.6 and PASW statistic version 18 software. We assessed explanatory power in a forwards stepwise manner, by examining the increase in variation explained when a new covariate or set of covariates were added to the existing model, together with a p-value for that increase.