QuantiGene 2.0 Reagent System was used and the protocol in the QuantiGene 2.0 Reagent System User Manual was followed with the exception of the substrate step. Lumigen® Lumi-Phos® Plus and 10% lithium lauryl sulfate was used instead of Lumigen® APS-5 substrate. A Biotek ELx 405 select plate washer was used for all of the wash steps in the assay. The QG 2.0 plates were then read on a Molecular Devices LMax luminometer with the plate incubator set to 45°C to maintain the temperature of the Lumigen® Lumi-Phos® Plus substrate. In total, 13 QG 2.0 plates were run to cover all target genes and the house keeping genes. Each house keeping gene ribosomal protein, large, P0 and ubiquitin C was loaded in duplicates at 12.5 ng/well. In addition, target genes (LRRK2, SCN8A and MAPT) were loaded in duplicates at 75 ng/well.
