To identify genes that were spatially or temporally regulated, we employed a conservative threshold (FDR q-value<0.01 and ≥2-fold log2-transformed signal intensity difference), included PMI and RIN as technical covariates within all of our ANOVA models of differential expression, considered the influence of dissection variation, and applied a 5-fold jackknife procedure (Supplementary Information 6.4; Supplementary Figs 12 and 13). We found that 70.9% of expressed genes were spatially DEX between any two regions within at least one period, and 24.1% were spatially DEX between any two NCX areas (Fig. 1a). In contrast, 90.0% of expressed genes were temporally DEX between any two periods across regions, and 85.3% were temporally DEX between any two periods across NCX areas. Moreover, 70.0% and 23.9% of expressed genes were both spatially and temporally DEX within brain regions and within NCX areas, respectively. The bulk of spatiotemporal regulation occurred during prenatal development. For instance, 57.7% of NCX-expressed genes were temporally DEX across fetal development (periods 3-7), in contrast to 9.1% during postnatal development (periods 8-12) and a mere 0.7% during adulthood (periods 13-15). Together, these data
