Following transplantation and rmuFGF21 treatment in Tg and WT mice Pparg and Fgf21 expression was measured (Figure 5 A–B). Fgf21 expression was increased in liver and white and brown adipose tissues (WAT and BAT) 10–25 fold in Tg mice compared to WT mice. Transplantation reduced liver Fgf21 expression in Tg mice, as did treatment with rmuFGF21 (Figure 5A). Treatment with rmuFGF21 had no effect on expression of endogenous Fgf21 in adipose tissues. However it led to a slight increase in expression of the transplanted adipose. Pparg expression was increased in the livers of Tg mice compared to WT mice and transplantation reduced liver Pparg expression in Tg mice in a similar manner to rmuFGF21 treatment, revealing a similar trend to hepatic Fgf21 expression (Figure 5B). Pparg expression in adipose tissues on the other hand were in opposition to those patterns observed for Fgf21; Pparg was approximately 50% lower in Tg adipose tissues compared to WT mice. Treatment with rmuFGF21 increased the expression of Pparg in transplanted adipose only.
