Braak stage [26] and atrophy were assessed by pathologists at McLean Hospital (Belmont, MA). Only neuropathologically confirmed AD subjects with Braak >3 were included in this profiling experiment. The profiled brain regions included dorsolateral prefrontal cortex (PFC, Brodmann area 9), visual cortex (VC, Brodmann area 17), and cerebellum (CR). These regions were chosen because PFC is impacted by the AD pathology while the latter two regions remain largely intact throughout most of the disease [26]. The tissue samples from subjects with HD diagnosis, while not the main focus of this work, were included to compare the two neurodegenerative diseases. The samples were flash frozen in liquid nitrogen vapor with an average postmortem interval (PMI) of about 18 hours. The clinical and demographic information for this study, including diagnosis, gender, age at the time of death, PMI, and Braak stage, is summarized in Table S1.
