et al., 2017; Yang et al., 2011). Alternatively, directed differentiation of hiPSCs involves long-term cultures with differentiation-specific growth factors and is generally thought to more closely resemble in vivo conditions but also to produce heterogeneous cell types (Maroof et al., 2013). This approach is suitable for exploring developmental components of AUDs in a mixed excitatory and inhibitory neuronal population. By contrast, induction of cell type specific genes significantly reduces the timeline and increases homogeneity of cell populations (Ho et al., 2016). We summarize some of these protocols in Table 2. Thus one advantage of hiPSCs is the availability of different reprogramming methods which allows the examination of the same genotype in different contexts.
