Human imaging, postmortem analyses, and animal studies of substance use disorders have demonstrated region-specific changes in neurons, astrocytes, oligodendrocytes, and microglia (for reviews, see Niciu et al., 2014; Zahr and Pfefferbaum, 2017). A significant advantage of hiPSC models is the expanding availability of various CNS cell types that can be derived from patients. This offers a valuable toolbox for exploring human cellular phenotypes within a specific genotype. A variety of reprogramming methods exist for generating specific CNS cells from hiPSCs, including direct induction of hiPSCs (Ahn et al., 2016; Close et al., 2017; Livesey et al., 2016; Lu et al., 2016; Muffat et al., 2016; Swistowski et al., 2010; Tcw et al. 2017b; Yu et al., 2014), neural progenitor cells (NPCs) (Ehrlich et al., 2017; Ho et al., 2016) or fibroblasts (Ladewig et al., 2012; Pang et al., 2011; Vadodaria et al., 2017; Yang et al., 2011). Alternatively, directed differentiation of hiPSCs involves long-term cultures with differentiation-specific growth factors and is generally thought to more closely resemble in vivo conditions but also to produce heterogeneous cell types (Maroof et al.,
