Although the mean CVs across the five CSF ROIs was lower with the sequential (2.1%) than independent (2.9%) method, this difference was not significant (Z=.405, p=.6858). Supratentorial volume showed a greater increase with the sequential method (mean±SD=12.23±13.11 cc, p=.0001) and lower CV (10.7%) than with the independent method (6.36±12.06 cc, p=.0095; CV=18.9%).